AKTIVITAS ANTIBAKTERI EKSTRAK JINTAN HITAM (NIGELLA SATIVA) TERHADAP BAKTERI STAPYLOCOCCUS AUREUS (ATCC)
DOI:
https://doi.org/10.36911/pannmed.v21i1.2567Keywords:
BlackCumin Extract, Staphylococcus aureus, Antibacterial Activity, Inhibition ZonneAbstract
Dental and oral health problems, including caries and plaque, are caused by bacterial infections such as Staphylococcus aureus. This bacterium plays a role in the formation of plaque biofilm, which can lead to caries if not controlled. One approach to control that is being developed is the use of natural ingredients with antibacterial activity. Black cumin (Nigella sativa) contains active compounds like flavonoids, saponins, tannins, and alkaloids, which have potential as antibacterials. This study aimed to determine the antibacterial activity of black cumin ethanol extract against the growth of Staphylococcus aureus (ATCC) bacteria. The research used an experimental method with a post- test only control group design. The extract was obtained through maceration with 96% ethanol and tested at three concentrations: 25%, 50%, and 75%. Chlorhexidine was used as a positive control, and Dimethyl Sulfoxide (DMSO) as a negative control. The study results indicated that black cumin ethanol extract (EEJH) showed comparable potential to chlorhexidine starting from a 50% concentration. However, increasing the concentration to 75% did not show a greater increase in antibacterial potential compared to the positive control group (chlorhexidine). In conclusion, black cumin (Nigella sativa) ethanol extract was able to significantly inhibit Staphylococcus aureus bacteria at a 25% concentration.
References
Arina, Y., Pratiwi, G., & Alta, U. (2023). Efektivitas Kombinasi Ekstrak Daun Sirih Hijau (Piper betle) Dan Daun Mint (Mentha piperita) Pada Uji Daya Hambat Bakteri Staphylococcus aureus. Jurnal ’Aisyiyah Medika, 8(2), 26–41
Arlandi, C. B. (2020). Hubungan Karies Gigi dengan Kejadian Endokarditis. Jurnal Medika Hutama, 2(1), 402–406.
Astika, F., & Muflihah, C. H. (2023). AKTIVITAS ANTIBAKTERI EKSTRAK DAN FRAKSI JINTAN HITAM (Nigella sativa) TERHADAP BAKTERI
Shigella sonnei DAN Bacillus cereus SERTA BIOAUTOGRAFINYA. Usadha Journal of Pharmacy, 2(1), 45–59. https://doi.org/10.23917/ujp.v2i1.112
Ayu, Z. P., & Pintadi, H. (2020). Daya Antibakteri Ekstrak Jintan Hitam dan Daun Sirih terhadap Staphylococcus aureus pada Plat Gigi Tiruan dilakukan dengan cara perendaman gigi Jintan hitam ( Nigella sativa ) digunakan oleh orang Negara Timur Tengah sebagai obat. Insisiva Dental Journal: Majalah Kedokteran Gigi Insisiva, 9(1), 19–25.
Beno, J., Silen, A. ., & Yanti, M. (2022). No. Braz Dent J., 33(1), 1–12. Endriani, R., Rafni,
