Diastase Enzyme Activity as a Quality and Clinical Safety Parameter of Pure Honey in Pharmacies in the Pasar Kemis
DOI:
https://doi.org/10.36911/pannmed.v21i1.2553Keywords:
pure honey, diastase enzyme, consumer safetyAbstract
Diastase enzyme is a natural constituent of honey highly sensitive to heat and storage, making its activity a primary indicator for bio-integrity and authenticity. This study aims to evaluate diastase activity in pure honey at pharmacies to ensure compliance with quality standards and consumer safety. This laboratory experimental study was conducted in Pasar Kemis and the Pharmaceutical Chemistry Laboratory of Universitas Indonesia Maju. The population included all packaged pure honey in pharmacies, using purposive sampling for five brands (samples A, B, C, D, and E). Instruments used included a spectrophotometer at 660 nanometers, with simple linear regression to determine the Diastase Number. Analysis revealed significant differences in enzymatic activity between samples. Sample A demonstrated optimal quality (7.49 DN), while samples B (0.12 DN), C (0.15 DN), and D (2.53 DN) were far below the minimum threshold of 3 units as per Indonesian National Standard 8664:2024. This decrease reflects molecular protein denaturation that is visually undetectable but implies therapeutic loss and toxic degradation risk. There is a significant anomaly between marketing authorization legality and biological quality in retail, necessitating systemic strengthening of post-market surveillance by regulatory authorities.
References
Moldakhmetova G, Toishimanov M, Nuraliyeva U, Khalykova G, Shkryl A. Evaluation Of The Physical And Chemical Characteristics Of Natural Honey Collected In Various Regions Of Kazakhstan. Sci Educ. 2025;6(3(80)):44-52.
Badan Standarisasi Nasional. SNI 8664:2024: Madu. Jakarta: BSN; 2024.
Badan Pengawas Obat dan Makanan Republik Indonesia. Laporan Tahunan Kedeputian Bidang Pengawasan Pangan Olahan. Jakarta: BPOM RI; 2021.
Yasin FM, Afriadi D, Qutni IA, Algeri EF, Karepoan MA. Penentuan Aktivitas Enzim Diastase dan Kadar Air Madu Hutan Asal Pulau Sula, Maluku Utara. J Biosains Pascasarj. 2025;27(1):1-4.
Pasias IN, Kiriakou IK, Proestos C. HMF and diastase activity in honeys: A fully validated approach and a chemometric analysis for identification of honey freshness and adulteration. Food Chem. 2017;229:425-31.
Mohammad NZ, Abdoulrahman K, Karim AY. Diastase enzyme activity and hydroxymethylfurfural production during thermal processing of honey. Diyala Agric Sci J. 2023;15(2):1-10.
Da silva Da Silva PM, Gauche C, Gonzaga LV, Costa ACO, Fett R. Honey: Chemical composition, stability and authenticity. Food Chem. 2016;196:309-23.
Winarno FG. Kimia Pangan dan Gizi. Jakarta: Gramedia Pustaka Utama; 2004.
Damodaran S, Parkin KL, Fennema OR, editors. Fennema's Food Chemistry. 4th ed. Boca Raton: CRC Press; 2007.
Sudarmadji S, Haryono B, Suhardi. Prosedur Analisa untuk Bahan Makanan dan Pertanian. Yogyakarta: Liberty; 1997.
Nelson DL, Cox MM. Lehninger Principles of Biochemistry. 5th ed. New York: WH Freeman and Company; 2008.
Damodaran S, Parkin KL, editors. Fennema's Food Chemistry. 5th ed. Boca Raton: CRC Press; 2017.
Ramawat KG, Mérillon JM, editors. Natural Products: Phytochemistry, Botany and Metabolism of Alkaloids, Phenolics and Terpenes. 2nd ed. Berlin: Springer; 2026.
Dewick PM. Medicinal Natural Products: A Biosynthetic Approach. 3rd ed. Chichester: John Wiley & Sons; 2009.
Harborne JB. Phytochemical Methods: A Guide to Modern Techniques of Plant Analysis. 3rd ed. London: Chapman and Hall; 1998.
Zakka GQ, Totong J. Penilaian Kualitas Madu Berdasarkan Parameter Hidroksimetilfurfural, Total Padatan Terlarut, Dan Kadar Air Di Beberapa Apotek Kabupaten Pasar Kemis Tahun 2025: Tinjauan Klinis Terhadap Keamanan Konsumen. J Ilm Farm Indones (JIFIN). 2025.
Srinivasan S, Murali R, Vinothkumar V, Raajasubramanian D, Selvaraj P. Therapeutic potential of honey against hepatotoxicity and renal toxicity: Insight into its mechanisms of action. Environ Sci Pollut Res. 2024.
Shapla UM, Solayman M, Alam N, Khalil MI, Gan SH. 5-Hydroxymethylfurfural (HMF) levels in honey and other food products: Effects on bees and human health. Chem Cent J. 2018;12(1):35.
Sari CR, Jaya F, Perdana RS, Riswantini D, Sustika R, Susilo A. Optimization of Honey Powder Formulation, Chemical Quality, and Antioxidant Activity Using Adaptive Feature Selection Based Multitarget Learning Approach. BIO Web Conf. 2026;218:03008.
Belitz HD, Grosch W, Schieberle P. Food Chemistry. 4th rev. and extended ed. Berlin: Springer; 2009.
Nelson DL, Cox MM, Hoskins AA. Lehninger Principles of Biochemistry. 8th ed. New York: W.H. Freeman; 2021.
Inda AR, Rosmiati M. Evaluasi Kepatuhan Label Produk Minyak Kelapa Murni Terhadap Peraturan BPOM Dan Standar Nasional Indonesia (SNI). J Teknol Dan Sains Modern. 2025;2(5):212-19.
