Perbedaan Kadar Hemoglobin pada Komponen Whole Blood Sebelum dan Sesudah Disimpan Selama 5 Hari di UTD PMI Kota Medan
Perbedaan Kadar Hemoglobin pada Komponen Whole Blood Sebelum dan Sesudah Disimpan Selama 5 Hari di UTD PMI Kota Medan
Keywords:
hemoglobin, whole blood, penyimpanan darahAbstract
Blood storage plays a crucial role in maintaining blood availability for transfusion, but it can impact the quality of blood components, including hemoglobin levels. This study aimed to determine the differences in hemoglobin levels in whole blood components before and after 5 days of storage at the Indonesian Red Cross (PMI) UTD in Medan City. The study used an observational analytical design with a quantitative approach to 41 whole blood samples selected using a simple random sampling technique. Hemoglobin levels were measured using a Sysmex XP-300 Hematology Analyzer. Data analysis was performed using the Shapiro–Wilk normality test and paired sample t-test. The results showed that the average hemoglobin level increased from 13.422 g/dL before storage to 14.598 g/dL after storage. The paired sample t-test showed a p-value <0.001, indicating a significant difference between hemoglobin levels before and after storage. The 8.76% increase in hemoglobin levels is suspected to be related to erythrocyte hemolysis during storage. It was concluded that 5 days of whole blood storage causes an increase in hemoglobin levels, so storage time and conditions need to be considered to maintain blood quality and the accuracy of laboratory test results.
References
Maharani EA, Noviar G. Imunohematologi dan bank darah. 2018.
Yusniati. Pengaruh Variasi Waktu Inkubasi Terhadap Kadar Hemoglobin Metode Drabkin’s Dengan Mikrolab 300. 2019.
Riki Rinaldi, Silvia Indra, Tasya Nadira Annisa. Perbedaan Hasil Pemeriksaan Kadar Hemoglobin Sampel Darah Vena Remaja Segera Diperiksa dan Ditunda dengan Metode Sahli. Jurnal teknologi Kesehatan Borneo. 2023 Dec 31;4(2):49–57. doi:10.30602/jtkb.v4i2.281
Fdil N. Blood Physiology and its Functions. Journal of Contemporary Medical Education. 2022.
Agesti N, Damrah D, Rasyid W, Welis W, Rahman D, Zarya F. The Role of Hemoglobin in Maintaining Health: A Literature Review. JURNAL INFO KESEHATAN. 2024 Jun 30;22(2):395–408. doi:10.31965/infokes.vol22.iss2.1482
Utami, Aprilia. Pengaruh Lama Simpan terhadap Jumlah Eritrosit pada Sediaan Whole Blood di Bank darah RSUD Bendan Pekalongan. [Internet]. 2017. Available from: http://repository.unimus.ac.id
Permenkes No 91. PERATURAN MENTERI KESEHATAN REPUBLIK INDONESIA. 2015.
Aliviameita A. BUKU AJAR MATA KULIAH IMUNOHEMATOLOGI UMSIDA PRESS Sidoarjo PROGRAM STUDI TEKNOLOGI LABORATORIUM MEDIS FAKULTAS ILMU KESEHATAN UNIVERSITAS MUHAMMADIYAH SIDOARJO 2020. 2020.
Saputro AA, Lestari CR. PENGARUH WAKTU PENYIMPANAN DARAH TERHADAP KADAR HEMOGLOBIN PADA KOMPONEN WHOLE BLOOD DARAH DONOR. JURNAL ANALIS LABORATORIUM MEDIK. 2021 Dec 21;6(2):50–6. doi:10.51544/jalm.v6i2.2066
Sarwoko, Ferdi. Pengaruh Lama Penyimpanan Terhadap Kadar Kalium Plasma Pada Whole Blood di UTD Dinas Kesehatan Mesuji. 2022.
Martha Adiratna N, Nelma, Analis Kesehatan J, Kesehatan Kemenkes Medan PR. PENGARUH PENYIMPANAN DARAH TERHADAP KADAR HEMOGLOBIN PADA KOMPONEN WHOLE BLOOD DARAH DONOR SEBELUM DAN SESUDAH DISIMPAN SELAMA SATU MINGGU DI PMI KOTA MEDAN. JoIMedLabS. 2023;4(1):70–7.
Suminingsih, Ariyadi, Tulus. Pengaruh Lama Simpan Kantong Darah Donor Pada Suhu 2-6 o Terhadap Kadar Hemoglobin Sebelum Transfusi Darah. 2017.
Susila S, Helin T, Joutsi-Korhonen L, Lauronen J, Ilmakunnas M. Quality of whole blood stored in room temperature for up to 5 days. Transfusion (Paris). 2025 May 1;65(S1):S193–203. doi:10.1111/trf.18133 PubMed PMID: 39908257.
Rahmah, Chairunissa. PENGARUH LAMA PENYIMPANAN KANTONG DARAH TERHADAP KADAR HEMOGLOBIN PADA KOMPONEN WHOLE BLOOD DI UNIT DONORDARAH PMI KOTA PALANGKA RAYA. 2021.
Avrilia Ranika Puteri O, Setyaji Y, Setyowatiningsih Politeknik Kesehatan Kementerian Kesehatan Semarang L. Hemoglobin Levels in PRC Blood Products Before and After Shelf Life Period. Academic Hospital Journal. 2025. Available from: www.journal.ugm.ac.id/ahj
