ACUTE TOXICITY TEST OF BENTONITE-PURIFIED FISH OIL IN WISTAR RATS (Rattus Norvegicus): HISTOLOGICAL, SGOT, AND SGPT STUDIES

Abstract

Purifying fish oil boosts its nutritional and economic worth. This study examined the acute toxicity of bentonite-purified eel and mackerel tuna fish oil (2, 4, 6%) on female Wistar rats (Rattus norvegicus), using a 5000 mg/kg fatal dose limit test, along with histological alterations, SGOT, and SGPT studies. A quasi-experimental post-test-only control was designed using one-way ANOVA and post hoc Tukey HSD tests. All animal groups experienced a steady weight gain from 155 ± 4.966555 g to 194.25 ± 8.421203 g on day 15. EBIII treatment revealed the greatest SGPT value of 76.33 ± 21.733 u/L, while MTBIII showed the highest SGOT value of 110 ± 20.881 u/L. One-way ANOVA results for SGPT (p > 0.05) revealed no significant change between treatment groups, although SGOT (p < 0.05) did differ significantly. A Tukey HSD post-hoc showed that each group's SGPT levels were not statistically different (p > 0.05). Compared to the normal group, the MTB 6% group exhibited a substantially different SGOT level (p < 0.05). The liver organ index increased in all treatment groups. Fatty degeneration and necrosis of glomeruli and tubules were visible in all treatment groups. The study found that pure fish oil bentonite did not harm Rattus Norvegicus.