Introduction Online antenatal care can help provide relatively economical medical services and reduce inequality for health checkups, especially for pregnant women, cost-effectiveness, especially in developing countries or large areas such as Indonesia, especially Dairi as a mountainous area where health services still cannot reach the entire community, especially in areas that do not have infrastructure and facilities that cannot be passed by vehicles such as motorcycles and cars. However, some pregnant women doubt the reliability of the online information because they do not communicate directly face-to-face. There fore, it was important to ensure the quality and security of online services. Online pregnancy examination services using an application so that midwives and patients can write complaints in the application where the 14 T service was already available in the application. Method: This study was quasi-experimental research. The sample was 84 midwives (Slovin formula) and was selected in a balanced manner (proportional to size). Bivariate analysis uses the paired t test to analyze the difference in midwifery knowledge before and after the trial of using the pregnancy examination application. Results of the study: Knowledge shows that before the trial of using the Samsider-Juli pregnancy examination application, the majority of midwives had poor knowledge, which was 48 people 57.1%. After the trial of using the Samsider – Julipregnancy examination application, the majority of good knowledge was 56 people 66.7%. The test using the paired t test showed that there was a difference in the knowledge of midwives before, namely the mean value of 30.15 and after the mean value of 31.35. Midwifery knowledge has increased after the trial of using the Samsider-Juli pregnancy examination application with a mean difference of 1.20238 and p value of 0.002. This means that statistically there was statistically significant difference (p<0.05) difference in knowledge before and after the trial of the pregnancy screening application. Conclusion: There was a change in the knowledge of midwives before and after the trial of the Samsider – Juli pregnancy examination application where the knowledge of midwives became better.